Part:BBa_K2666007
RFP with signal peptid YncM with a L. jensenii specific strong promoter
The Montpellier iGEM team 2018 designed a construction that produce and secrete YLP20, a nanobody against YLP12 in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is B. subtilis specific [2]. Figure 1 illustrates the detailed design.
Figure 1 : Construct design. The sequence that encode RFP with a SP YncM and produce by a strong promoter RpsU.
YLP20 is This ASA targets the YLP12 antigen that is present on spermatozoa. It inhibits sperm zona binding and motility of spermatozoa [3]
Proof of function Test anti-microbien ?
Sequence and feature seq on benchling
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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