Coding

Part:BBa_K2653004

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-10)


HA Tag

We use this part to tag our trim21 so that it can be easily detected.


Usage and Biology

HA-tag has long been used as the epitopic tag when it is hard to test whether a target gene has expressed or not. The HA-tag we use in our system has the length of 27 bps. By testing HA-tag with its antibody, we successfully validated the expression of trim21 which has hardly no epitopic sign to test. It is a really useful tool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None