Composite

Part:BBa_K2638022:Design

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Moderate constitutive expression system for OprC and CopD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1091
    Illegal NheI site found at 1114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1397
    Illegal NgoMIV site found at 1728
    Illegal NgoMIV site found at 1932
    Illegal NgoMIV site found at 2976
    Illegal NgoMIV site found at 3177
    Illegal AgeI site found at 1391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 326


Design Notes

Biobrick Assembly by restriction and ligation.


Source

Genome of Pseudomonas brassicacearum 3Re2-7 13 and iGEM Distribution Plates from 2018

References