Coding

Part:BBa_K2637003:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)


KaiC Protein of Circadian Rhythm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 262
    Illegal BglII site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.

Source

KaiC is from the the cyanobacterium Synechococcus elongatus PCC7942 and is the core of circadian clock because Kai system’s circadian rhythm is associated with KaiC autophosphorylation and dephosphorylation.

References