Coding

Part:BBa_K2568017:Design

Designed by: Ying Xu   Group: iGEM18_Jiangnan   (2018-10-07)


Virus receptor TfR with Kozak sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added kozak sequence to enhance translation efficiency and protein expression.


Source

We extracted the total RNA of MDCK cells, used TAKARA reverse transcription kit to reverse transcribe RNA and obtained cDNA. Using cDNA as a template, we amplified TfR by PCR.

References