Tag
Part:BBa_K2560086:Design
Designed by: Tobias Hensel Group: iGEM18_Marburg (2018-09-21)
Phytobrick version of 5a His-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGGCTTTACACCATCACCACCATCATGGGTA
Reverse Oligo: CTCATACCCATGATGGTGGTGATGGTGTAAAGC