Coding

Part:BBa_K2557004:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


PhiC31 recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 912
    Illegal BamHI site found at 998
    Illegal BamHI site found at 1245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 308
    Illegal NgoMIV site found at 350
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604
    Illegal BsaI.rc site found at 1007
    Illegal BsaI.rc site found at 1360


Design Notes

Since we used animal cells as the chassis organism, the coding sequence was humanized.


Source

We found the coding sequence of PhiC31 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References