Regulatory

Part:BBa_K2534007:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM18_AFCM-Egypt   (2018-09-20)


AMPR promoter + AMPR

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at # BBa_K2308011 by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids.

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's apoptotic effect of RKO cell lines.

ChAMPRAFCM.png


For further information about demonstration of its apoptotic effect, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 254


Design Notes

IDT syntheised


Source

IDT synthesised

References