Coding

Part:BBa_K2510009

Designed by: Oleksandra Sorokina   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


CARP1 regulated

This part is contain coral acid-rich protein1(CARP1, Part:BBa_K2510000) and regulated with T7 promoter, induced lac operator, RBS B0034 and T7 terminator. Coral acid-rich protein1(CARP1) is derived from expression of genes obtained from the common stony coral, Stylophora pistillata and was identified to be responsible for calcium carbonate precipitation. CARP1 contain a secretory signal peptide, suggesting that they are discharged from the cells. Also contains an EF hand Ca2+-binding domain.The putative mechanism of calcium carbonate nucleation is that the highly acidic pocket of CARPs localize the substrate, buffer and catalyze the reaction between calcium ion and carboxylate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None