Coding

Part:BBa_K2510005

Designed by: Pierre-Luc Satin   Group: iGEM17_Paris_Bettencourt   (2017-10-02)


Silicatein alpha/cellulose gene

This is an artificial gene. It is build on a basis of the cellulose producing protein and has the active part of the silicateinα protein. This construct also have a T7 promoter and an histidine-tag. The silicateinα part can precipitate silicate into polysilicate which is a reaction that would normally require a way higher temperature and pression.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 607
    Illegal EcoRI site found at 19
    Illegal XbaI site found at 85
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
    Illegal SpeI site found at 608
    Illegal PstI site found at 622
    Illegal NotI site found at 25
    Illegal NotI site found at 615
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
    Illegal BamHI site found at 13
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 608
    Illegal EcoRI site found at 19
    Illegal XbaI site found at 85
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 19
    Illegal XbaI site found at 85
    Illegal SpeI site found at 608
    Illegal PstI site found at 622
    Illegal NgoMIV site found at 250
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]



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