View source for Part:BBa K2448006


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<partinfo>BBa_K2448006 short</partinfo>

This part is an ''E. coli'' codon optimized version of the PsiR found in ''Agrobacterium tumefaciens'' str. C58 (Atu4743).

===Usage and Biology===

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG). 

Our team used this transcription factor together with the ''Agrobacterium tumefaciens'' pPsiA or pPsiR promoters ([[Part:BBa_K2448010|BBa_K2448010]] and [[Part:BBa_K2448011|BBa_K2448011]]) as well as with the pPsiTac1 synthetic promoter ([[Part:BBa_K2448016|BBa_K2448016]]) to build three Psicose biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]] and [[Part:BBa_K2448027|BBa_K2448027]]). The results show that PsiR behaved as predicted under and without induction.

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2448006 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2448006 parameters</partinfo>
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