Coding

Part:BBa_K2443062:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)


MTF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 839
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in Escherichia coli.



Source

uniprot.org

References

Histidine tag at C-terminus (6 histidine residues) with 18 amino acid linker sequence: http://pubs.acs.org/doi/pdf/10.1021/bi9926072