Part:BBa_K2433003

traAC

The traA and traC genes are part of the tra region of the native Ti plasmid of Agrobacterium tumefaciens . The traAC part is composed of the region between the traA and traAC coding regions and exhibits bidirectional promoter behavior (Faure and Lang, 2014). The tra region is regulated by the TraR protein when TraR is bound to Agrobacterium Autoinducer (AAI) (Piper et al., 1993). In turn, TraR is upregulated by opines, which are metabolites released by infected plant cells. (Fuqua and Winans, 1994). Therefore, this promoter is activated by a quorum of bacteria and a nearby infected plant cell. The TraAC promoter can be used to conditionally express a gene in cases of Agrobacterium infection of a plant.

References

Faure, D., & Lang, J. (2014). Functions and regulation of quorum-sensing in Agrobacterium tumefaciens. Frontiers in plant science, 5, 14.

Fuqua, W. C., & Winans, S. C. (1994). A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. Journal of bacteriology, 176(10), 2796-2806.

Piper, K. R., von Bodman, S. B., & Farrand, S. K. (1993). Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature, 362(6419), 448-450.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 81
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]