Composite

Part:BBa_K2389070:Design

Designed by: Rochelin Dalangin   Group: iGEM17_UAlberta   (2017-10-26)


GFP reporter under lac promoter + lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

Ensuring that the lacI promoter is strong enough to suppress GFP expression appreciably when cAMP is not present, but can be overpowered when cAMP is.


Source

GFP is from aequorea victoria, and lacI is from E. coli.

The inspiration for this part is K611016, except we changed the promoter to a constitutive promoter

References