Composite
Part:BBa_K238004:Design
Designed by: Annelien Verfaillie Group: iGEM09_KULeuven (2009-07-15)
Vanillin synthesis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 895
Illegal BamHI site found at 1297
Illegal BamHI site found at 6341 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 638
Illegal NgoMIV site found at 1682
Illegal NgoMIV site found at 1690
Illegal NgoMIV site found at 2440
Illegal NgoMIV site found at 2613
Illegal NgoMIV site found at 3015
Illegal NgoMIV site found at 3019
Illegal AgeI site found at 585
Illegal AgeI site found at 754
Illegal AgeI site found at 4929
Illegal AgeI site found at 5466
Illegal AgeI site found at 5927
Illegal AgeI site found at 6369 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 189
Illegal BsaI site found at 455
Illegal BsaI.rc site found at 2214
Illegal BsaI.rc site found at 3846
Design Notes
parts for fcs (BBa_I742114) and ech (BBa_I742112) will probably not be used and made from another sequence.
Source
most parts were found in the registry.