Intermediate

Part:BBa_K235017:Design

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[I0500][B0034] (pAra+RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Constructed using three-antibiotic assembly. On plasmid pSB1C3.


Source

I0500+B0034

References