Part:BBa_K2273002:Design

P_rapF: a ComA-dependent promoter of Bacillus subtilis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the rapF promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The rapF promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PrapF fwd RFC10	gatcGAATTCGCGGCCGCTTCTAGAGAAACATGCGGTGTAACGAAAG
PrapF rev RFC10	gatcCTGCAGCGGCCGCTACTAGTATCATAATTCTATATATGTCATATTTTATC

References

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed