Composite

Part:BBa_K2236002:Design

Designed by: Brendan Reiter   Group: iGEM17_UNBC-Canada   (2017-10-23)


lac inducible expression system for Staph. Hfq


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensure that there is a SpeI scar region between the RBS and start codon of Hfq to allow a proper spacer for protein expression


Source

Washington iGEM 2010, for the design of the expression system Hfq comes from Staphylococcus aureus ATCC 700699 genome: http://www.uniprot.org/uniprot/A0A0H3JV59

References