Coding

Part:BBa_K2165001

Designed by: Roya Amini-Naieni   Group: iGEM16_Washington   (2016-10-14)


Violacein D gene codon-optimized for S. Cerevisiae

VioD is the fourth (and final if VioC is not also present) enzyme in the violacein pathway when expressed. VioD, alongside VioA, VioB, and VioE, processes its substrate protodeoxyviolaceinic acid into protoviolaceinic acid. When VioC is unavailable, this degrades into proviolacein, a teal-colored pigment. When VioC is present this precursor is processed into an intermediate which subsequently degrades into a dark, purple pigment called volacein.

VioD was previously available as a standalone part as BBa_K539413, produced by NCTU Formosa's 2011 iGEM team; however, BBa_K539513 is not codon optimized for yeast. The part developed by the University of Washington's iGEM team have been codon optimized for yeast in hopes to improve the efficiency of its translation. The CDS contained in this BioBrick was designed by running the VioD gene sent to the University of Washington by the Dueber Laboratory at University of California-Berkley through IDT's Codon Optimization Tool for S. cerevisiae.

Usage and Biology

It should be stressed that without the other components of the Violacein pathway (VioA, VioB, and VioE, see part BBa_K726015 as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 622
    Illegal SapI.rc site found at 1003


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Categories
Parameters
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