Composite

Part:BBa_K2142005

Designed by: Lukas Rieder   Group: iGEM16_NAWI-Graz   (2016-10-07)


Composite part containing inducible mazF toxin and dam metylase

In our project we used this composite part to let our E.coli attack with the mazF toxin (BBa_K2142003). Further this part contains a Dam methylase (BBa_K2142004) for the reason this protein leads with over expression to an increased mutation rate. For the reason toxin production and mutation can cause problems during assembling and testing the expression of the proteins can be induced with IPTG. This part belongs to BBa_K2142000. The results for the working part are shown here http://2016.igem.org/Team:NAWI-Graz/Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None