Part:BBa_K2109104:Experience

We were unable to full assemble the bacterial-two-reporter cassette with the RNA polymerase (K2109106) to generate the final plasmid construct (K2109105). However, we tested the reporter cassette for functionality using flow cytometry with cytometer settings configured for measuring mRFP1 and mTagBFP fluorescence.

As evidenced by our flow cytometry results, we observed high mTagBFP fluorescence from E. coli DH5alpha cells containing pSB3K3 and the part, while a control strain without the part shows no detectable fluorescence for mTagBFP. Interestingly, we also observed a very small but detectable mRFP1 signal in cells containing the part with resepect to the control strain. Collectively, these results demonstrate a functional baseline expression of the two fluorescent proteins.

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