Composite
Fimh + RPM

Part:BBa_K2052017

Designed by: Burak Kızıl, Seniz Yuksel   Group: iGEM16_METU_HS_Ankara   (2016-09-26)


Fimh + RPMrel + dTerm + AraC + RBS + ButCoat

It is the same construct that we have in the part named as K2052016. The only difference between two parts is the backbone. This parts was synthesized into pUC57 cloning vector and sent us from GeneScript to use in our work.


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Figure 1.Circuit


Metusss.jpeg

Figure 2.Gel Image


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1173
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None