Coding

Part:BBa_K1972013:Design

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-14)


dszBAC with tac promoter and dszD with lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
    Illegal XhoI site found at 4243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 1761
    Illegal AgeI site found at 1104
    Illegal AgeI site found at 1865
    Illegal AgeI site found at 2148
    Illegal AgeI site found at 2966
    Illegal AgeI site found at 4431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 864


Design Notes

Not yet.


Source

Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)

References