Regulatory

Part:BBa_K1968021:Design

Designed by: Ricardo Camilo Chávez Martínez   Group: iGEM16_Edinburgh_OG   (2016-10-14)


Synechocystis Sca6-2 Promoter with RBS* (LacI repressible) Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Phytobrick Standard was used for this part


Source

Albers et al. Promoter Library

This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing

References

Albers, S.C., Gallegos, V.A. & Peebles, C.A.M., 2015. Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, pp.36–46.