Coding

Part:BBa_K1964001:Design

Designed by: Steffen Lütke   Group: iGEM16_Marburg   (2016-10-14)


Codon optimized GcABC-G1 for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2932
    Illegal BsaI.rc site found at 34


Design Notes

Biobrick cutsites had to be codon optimized for Saccharomyces cerevisiae and the biobrick cutsites had to be removed during sequencing.



Source

The original gene has been found in the genome of Grossmannia clavigera. The submitted sequence was synthesized by idtDNA and codon optimized for Saccharomyces cerevisiae.

References