Composite

Part:BBa_K1893006:Design

Designed by: Alyssa Henderson   Group: iGEM16_Imperial_College   (2016-10-14)


Cin receiver (CinR+pCin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 611
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not include an LVA tag on the CinR gene. This means that the protein is not rapidly degraded. When active, the CinR protein binds to the pCin promoter, activating transcription of downstream genes. This part contains everything necessary for O3C14-HSL-induced expression of genes inserted downstream of the pCin promoter.


Source

The CinR part was synthesised by GeneArt by Thermofisher and assembled using Biobrick assembly with the other components.

References