Composite

Part:BBa_K1824002:Design

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)


Constitutive Promoter J23119 + RNA Thermometer U6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 46
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence.


Source

J23119 and U6 were from the Registry.

References