Part:BBa_K176192:Design

PoPS->GFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2396
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 662
  

Design Notes

Input: PoPS

Output1: GFP

Output2: LuxI-LVA

Output3: PoPS

Design:

Input PoPS generates Output1 GFP and CI-LVA

pCI generates Output2 LuxI-LVA and Output3 PoPS, repressed by CI-LVA

Source

I13500 K176189