DNA

Part:BBa_K1560001:Design

Designed by: Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick   Group: iGEM14_UCSC   (2014-09-27)


pSCKiKo Knock-In Knock-Out Vector Backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 947
    Illegal NgoMIV site found at 1496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1019


Design Notes

Backbone contains a region for Gibson cloning.


Source

Modified pTA963 plasmid, originally developed by Thorston Allers (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838008/)

References