Project

Part:BBa_K153008:Design

Designed by: Dmitry O. Malyshev   Group: iGEM08_University_of_Sheffield   (2008-10-29)

CqsA - the CAI-1 producer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1191
    Illegal SpeI site found at 1217
    Illegal PstI site found at 1203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1191
    Illegal SpeI site found at 1217
    Illegal PstI site found at 1203
    Illegal NotI site found at 1208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1191
    Illegal BglII site found at 89
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1191
    Illegal SpeI site found at 1217
    Illegal PstI site found at 1203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1191
    Illegal SpeI site found at 1217
    Illegal PstI site found at 1203
    Illegal AgeI site found at 404
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene produces is responsibe for production of the CAI sensing molecule. It is normally induced by IPTG. Note due to the sort half life of CAI at temperatures above 25C[1], it is reccomended to grow the cells a room temperature.






[1] The Major Vibrio cholerae Autoinducer CAI-1 Supplementary Information" Nature 450, 883-886 (6 December 2007) by Douglas A. Higgins, Megan E. Pomianek, Christina M. Kraml, Ronald K. Taylor, Martin F. Semmelhack & Bonnie L. Bassler

Source

to be added

References