Endo-1,4-beta-glucanase (cellulase gene)
Endo-1,4-&-beta-glucanase (EG 1) is an enzyme that breaks down the 1,4-beta-D-glycosidic linkages in cellulose to produce glucose. The coding sequence of this gene was from Neisseria Sicca bacterium that uses cellulose acetate as its sole carbon source. This enzyme is specific for 1,4-beta linkages in cellulose. The molecular mass of EG 1 is 50kDa and its isoelectric point is 5.0. EG 1 contains a carbohydrate binding module at its N-terminus.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 313
- 1000COMPATIBLE WITH RFC
1. Moriyoshi K et al. (2003) Role of endo-1,4-beta-glucanases from neisseria sicca SB in synergistic degradation of cellulose acetate. Biosci Biotechnol Biochem. 2003 Feb;67(2):250-7/ Link: www.ncbi.nlm.nih.gov/pubmed/12728982
2. Sakai K et al. (1996) Biodegradation of cellulose acetate by Neisseria sicca. Bioscience, Biotechnology, Biochemistry 10: 1617-1622. PMID: 8987659.
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