Part:BBa_K1486056:Design
CxpR & Split IFP1.4 [Cterm + Cterm][2]
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3673
Illegal BamHI site found at 1144
Illegal XhoI site found at 1297
Illegal XhoI site found at 2473 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1708
Illegal AgeI site found at 2884 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Site-directed mutagenesis was used to remove the restriction sites in the sequence. It is to be noted that this construct makes use of an Arabinose-inducible promoter, a standard Elowitz RBS, as well as a flexible linker 2 x (Gly-Gly-Gly-Gly-Ser) between CpxR and the two split parts of the IFP WARNING: It is also to be noted that basic parts are submitted with a start codon and a stop codon. To make a composite part, START and STOP codons of the basics parts may have been removed, as we only want a START codon at the beginning of a cds and a STOP codon at the end of a cds. You should consider it when looking at the part sequence.
Source
http://www.addgene.org/browse/article/8177/ which was then mutated by us.
References
Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.