Composite

Part:BBa_K1463701:Design

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-10-04)

MotA and B0032 RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 80
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 848


Design Notes

To make the RBS + motA biobrick, motA was amplified by PCR using the proofreading Phusion polymerase using DS941 genomic DNA as template. The forward primer incorporated the prefix, added the BBa_B0032 ribosome binding site (RBS) and a scar sequence just upstream of motA and changed the natural GTG start codon to ATG. The reverse primer incorporated the suffix and changed the stop codon to TAA.

800px-GU_MotA_primer.png