Intermediate

Part:BBa_K1459004:Design

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-01)


PmrA-PmrB(LBT) with terminator (BBa_B1006)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 692


Design Notes

Not many, apart from ligating these two parts together.


Source

PmrA-PmrB operon sequence was obtained thanks to kindness of prof. He from the University of Chicago. BBa_B1006 was obtained from 2014 Spring Distribution.

References