Coding

Part:BBa_K1456026

Designed by: Mustafa Yılmaz   Group: iGEM14_ATOMS-Turkiye   (2014-10-23)

tetR DNA Binding Domain

  • The effector is a tetracycline-controlled transactivator (tTA) of transcription that consists of a chimeric construct of the Escherichia coli Tn10 tetR gene (purple) and the VP16 transactivation domain (orange). In the absence of the inducer, doxycycline (Dox), tTA dimers specifically bind to seven tandemly repeated 19-bp tetO sequences (tetO7), thereby activating transcription from a minimal promoter (TATA) and driving expression of the target transgene that encodes the gene of interest (target ORF). When bound to Dox, tTA undergoes a conformational change and cannot bind tetO sequences.
ATOMS tetR.gif.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Nature Reviews Genetics 2, 743-755 (October 2001) | doi:10.1038/35093537


[edit]
Categories
Parameters
n/atetR DNA Binding Domain