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Part:BBa_K145005:Design
Designed by: Jonas Demeulemeester Group: iGEM08_KULeuven (2008-07-08)
T7 PoPS + PR -> cI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 822
Design Notes
Verify the dual promotor. Check if transcription can still proceed from the T7 promotor when cI is bound.
Source
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