Plasmid

Part:BBa_K1441012:Design

Designed by: Derrica Burke   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase from Escherichia coli with His-tag In pGAPz alpha A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

The original part in the PSB1C3 vector had to be altered using PCR in order to remove the stop codons present in the IGEM standard prefixes and suffixes.


Source

This part comes from a standerized IGEM biobricks PSB1C3 plasmid.

References