Measurement

Part:BBa_K1369013:Design

Designed by: Galen Gao   Group: iGEM14_Caltech   (2014-10-17)


lamBD_3xFLAG_N


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 99
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 258
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 99
    Illegal AgeI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inserted 3xFLAG at N-terminus (5' end) of lamD, which is the prepeptide of the signaling ligand. Assembled via Gibson assembly. Not biobricked.


Source

Lactobacillus Plantarum

References