Generator

Part:BBa_K1361005:Design

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-06)


CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CsgEFG was PCRed as a whole. The EcoRI loci within CsgA sequence has been replaced from GAATTC to GAATTT.



Source

PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.

References