Part:BBa_K1335001:Design
Vh p53 + Ig hinge + his tag + HlyA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
GTTTCTTCGAATTCGCGGCCGCTTCTAGatggaggtgcagctggtggagtctgggggaggcttggtgcagcctggggggtctctgagactctcctgtgcagcctctggattcaccttcgatggtttccggatgtattgggtccgtcaggcaccggggaaggggctcgagtgggtctcggctattaatacgagtggtcgcaccacatactatgcagactccgtgaagggccgattcatcatctccagagacaacgccaagaacacgctgtatctgcaaatgaacagtctgaaatctgaggacacggccgtgtattactgcgcaaaagatggaggatactatagtcgtatgtactactttgggtactggggccaggggacccaggtcaccgtctcctcagcctcgggggccgcgtcgacgtccggcggtccgaagccttccactccgcccgggtcttcccgtatgaaacagctggaagacaaagtagaggagctccttagcaagaactaccatctcgaaaacgaggtagctcgtctgaaaaagcttgttggtgaacgtggtggtgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcaccatcaccatcaccatgcgtcgacgcccgggggtgcgccggtgccgtatccggatccgctggaaccggccggggaaaattctcttgctaaaaatgtattatccggtggaaaaggtaatgacaagttgtacggcagtgagggagcagacctgcttgatggcggagaagggaatgatcttctgaaaggtggatatggtaatgatatttatcgttatctttcaggatatggccatcatattattgacgatgaaggggggaaagacgataaactcagtttagctgatatagatttccgggacgttgcctttaagcgagaagggaatgacctcattatgtataaagctgaaggtaatgttctttctattggccacaaaaatggtattacatttaaaaactggtttgaaaaagagtcagatgatctctctaatcatcagatagagcagatttttgataaagacggcagggtaatcacaccagattctcttaaaaaagcatttgaatatcagcagagtaataacaaggtaagttatgtgtatggacatgatgcatcaacttatgggagccaggacaatcttaatccattaattaatgaaatcagcaaaatcatttcagctgccggtaacttcgatgttaaggaggaaagatctgccgcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataactttgacagcatcagcataaGTTTCTTCCTGCAGCGGCCGCTACTAGTA The gene is not in standard pSB1C3 plasmid but in pUC57 vector, but it contains standard prefix and suffix.
Source
Naturally, nanobodies are present in camelids family, however we decided to use the approach of bacterial production. The gene was synthesized by GenScript company.