Device

Part:BBa_K1260005:Design

Designed by: Zhengfeng Liu   Group: iGEM14_Shenzhen_SFLS   (2014-05-24)


pbad-ScFv (whole device)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A L-arabinose, a strong RBS and a double termintor has been added to the CDS. The expression of this device is very high, for the cds have been improved.

Source

It is constructed by four basic parts: BBa_K206001 BBa_B0030 BBa_K1260000 BBa_B0015.

References