Generator

Part:BBa_K1179074:Design

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-18)


Hef1a_eYFP-4x503


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2077
    Illegal SpeI site found at 2053
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
    Illegal PstI site found at 1961
    Illegal PstI site found at 1984
    Illegal PstI site found at 2007
    Illegal PstI site found at 2030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2077
    Illegal SpeI site found at 2053
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
    Illegal PstI site found at 1961
    Illegal PstI site found at 1984
    Illegal PstI site found at 2007
    Illegal PstI site found at 2030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2077
    Illegal BglII site found at 569
    Illegal BamHI site found at 1197
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2077
    Illegal SpeI site found at 2053
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
    Illegal PstI site found at 1961
    Illegal PstI site found at 1984
    Illegal PstI site found at 2007
    Illegal PstI site found at 2030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2077
    Illegal SpeI site found at 2053
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
    Illegal PstI site found at 1961
    Illegal PstI site found at 1984
    Illegal PstI site found at 2007
    Illegal PstI site found at 2030
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There will still be low yellow fluorescence background expression in the presence of miRNA503. It is recommended that people interested in using this part, test for this small amount of background expression before performing experiments with this part.

Source

Constructed by performing a gateway reaction with entry vectors Hef1A and eYFP-4x503ts. See components for more information

References