Composite

Part:BBa_K1173502:Design

Designed by: paul james   Group: iGEM13_Exeter   (2013-09-20)

ompC w/GFP + (BglII+BamHI)restriction sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BamHI site found at 877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 792


Design Notes

You MUST be concious of the BglII and BamHI cut sites within the construct. These enzymes are used for several assembly techniques, so these must not be applied when assembling the part with others.


Source

Synthesised by IDT (gBlocks). Based on a variety of BioBricks.

References