Regulatory
HIP
Part:BBa_K1166000:Design
Designed by: Luis Mario Leal Garza Group: iGEM13_TecMonterrey (2013-09-17)
FNR-HIP1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The FNR gene has additional nucleotides in the 5' direction including the 3' end of the ORF upstream in the genomic sequence. By producing the FNR protein, hypoxic regulation becomes stronger.
Source
FNR is a native E.coli gene that we had synthesized for our project. HIP1 promoter was also synthesized and belongs to Salmonella.