Regulatory
HIP

Part:BBa_K1166000:Design

Designed by: Luis Mario Leal Garza   Group: iGEM13_TecMonterrey   (2013-09-17)


FNR-HIP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The FNR gene has additional nucleotides in the 5' direction including the 3' end of the ORF upstream in the genomic sequence. By producing the FNR protein, hypoxic regulation becomes stronger.

Source

FNR is a native E.coli gene that we had synthesized for our project. HIP1 promoter was also synthesized and belongs to Salmonella.

References