Coding

Part:BBa_K1164002:Design

Designed by: Wilson Lam, Dylan Siriwardena   Group: iGEM13_uOttawa   (2013-09-16)


LacI repressor tagged with yBFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to remove the illegal XbaI site, site-directed mutagenesis was performed, changing nucleotide 255 from a thymine to a cytosine, and also changing nucleotide 258 from an adenine to a glycine.


Source

E.coli/commercial synthesis

References