Regulatory

Part:BBa_K112711:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


rbs.spvR!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of rbs.SpvR! basic part K112711

PCR of rbs.SpvR! al0027 and al0028   (933bp, EcoRI, BamHI, DpnI)
Sub into pBca1256                        (EcoRI/BamHI, 2472+933bp, L)
Product is pBca1256-K112711

------------------------------------------------
al0027 Forward Biobricking of rbs.spvR! CCATAGAATTCatgAGATCTAAAAAAGGAGATATTATGGATTTCTTG

al0028 Reverse Biobricking of rbs.spvR! CGttaGGATCCaggtcagaaggtggactg

References