Regulatory

Part:BBa_K112320:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-22)


{< ftsAZ promoter >} in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag


Source

MG1655

References