Composite

Part:BBa_K1111000:Design

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-18)


Hya promoter_GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1249


Design Notes

It was not very clear how long the promoter was, thus we took a ~500 bp long fragment upstream from the beginning of the gene that is originally controlled by this promoter


Source

E.coli, substrain MG 1655

References