Reporter

Part:BBa_K1100016

Designed by: Chenxi He, Jiadong Ni   Group: iGEM13_Fudan   (2013-09-13)

R0040 with insulator and sfGFP

We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
absB0034
biology
controlaTc, tetracyline
directionForward
emission510nm
excitation485nm
negative_regulators1
o_h
o_l
positive_regulators
proteinsfGFP