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Part:BBa_K1059001:Design

Designed by: Yu Wang   Group: iGEM13_OUC-China   (2013-09-17)


GFP-LVA under J23101 control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 709


Design Notes

The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR. See data at http://2013.igem.org/Team:OUC-China/RNA_guardian/Results

Source

The GFP-LVA sequence is from part 145280, but we change the RBS.

References